Scientific Tool

LC Gradient Designer

Create optimized liquid chromatography gradient profiles using the Linear Solvent Strength model. This easy-to-use web tool simplifies gradient design, providing reproducible parameters and deeper insight into gradient behavior. Generate and copy a formatted gradient table instantly—no login or data storage required.

System Parameters

Column Length (cm)

Column ID (mm)

Particle Type

Column Dead Volume (V_m):1.13 mL

How To Determine This Value?

Advanced Parameters

These values enable additional validation warnings and calculations to help optimize your gradient method.

Delay Volume (mL)Internal volume between gradient formation and columnHow To Determine This Value?

Void Volume (mL)Internal volume between Sample and Column at the moment of injectionHow To Determine This Value?

Injection Volume (µL)Typical injection volume for your gradient

Injection Cycle (min)Time delay between the end of a sample and the injection of the next sample in a sequence

Gradient Steps

Total Run Time: 0.00 min

How to use this table

Adding steps: Use the buttons below the table to add Isocratic Hold, Gradient Ramp, or Step Change segments.

Edit Values: Each row has an "Edit Values" dropdown that determines which parameters are user-defined versus calculated automatically.

Columns: F = Flow rate (mL/min), B_init/B_final = Initial/final %B, t_G = Duration (min), nV_m = Column volumes, k*S = Gradient steepness parameter.

Editing cells: Click any white cell to edit. Gray cells are calculated based on your Edit Values selection. Calculations are dynamic.

Deleting: Click the trash icon on any row.

Label

Type

Edit Values

B_init

B_final

t_G

nV_m

k*S

Start

End

No gradient steps defined.

Click one of the buttons below to add a step.

mL/min

Gradient Profile

Metadata

Cycle Time: 0.50 min

Time 'on-column' for full injection slug: 6.6 Seconds

Total Equilibration Column Volumes (with injection cycle): 0.00

Export Gradient Table

Copy the gradient data to paste into spreadsheets or instrument software.

Format:

Preview (Full Table)

# LC Gradient Designer Export
# Generated: 2026-02-02T14:10:31.567Z
#
# System Parameters:
# Column: 10 cm x 4.6 mm ID
# Particle Type: Fully Porous
# Flow Rate: 1 mL/min
# Vm (calculated): 1.13 mL
# Total Run Time: 0.00 min
#
Step	Label	Type	B_init (%)	B_final (%)	tG (min)	Start (min)	End (min)	nVm	k*S

Usage Tips

• Full Table: Complete data including all calculated values. Paste into Excel or Google Sheets.
• Time/%B Only: Simplified format for instrument gradient tables. Contains just the time and %B columns.
• Lines starting with # are comments and may be ignored by instruments.

Need Help or Have Ideas to Improve This Tool?

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